Exposure to cigarette smoke is one of the exogenous free radicals. Exposure tocigarette smoke is associated with lung inflammation, oxidative stress and lipidperoxidation. Compounds that can inhibit the oxidation reaction by binding free radicalsknown as antioxidants. Eleutherine americana Merr. leaves has an antioxidant activity ininvitro study. The aim of this work was to study E.americana leaves as a naturalantioxidant that reduce the level of lung damage from exposure to cigarette smoke inmale wistar rats and study effective dose of E.americana leaves to reduce the level oflung damage. The leaves of E.americana extracted by maceration method for 6 daysusing ethanol 70%. Rats were divided into six groups of four animals each : exposed toambient air (GroupA), exposed to cigarette smoke (Group B), exposed to cigarette smokeand treated with ethanol extract of E.americana leaves at dose 45mg/kg bw, 90mg/kg bw,180mg/kg bw (Group C,D,E), exposed to cigarette smoke and treated with vitamin E dose 18mg/kg bb(Group F). All rats were treated for 14 days using 3 cigarettes non filteredeach day by using a smoking chamber. After 14 days rats were killed. Right lungs wereremoved for histopathology. The results showed that dose 45mg/kg bw, 90mg/kg bw,180mg/kg bw can reduce the level of lung damage to the enlarge of alveolar, thickeningof the alveolar wall and inflammatory cell infiltration. Dose 180mg/kg bw as an effectivedose to reduce the level of lung damage that compare to normal control.
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机译：接触香烟烟雾是外源性自由基之一。接触香烟烟雾与肺部炎症，氧化应激和脂质过氧化有关。通过结合称为抗氧化剂的自由基来抑制氧化反应的化合物。美国大叶黄杨。叶片在体外研究中具有抗氧化活性。这项工作的目的是研究作为一种天然抗氧化剂的美国大叶E.americana叶片，它可以降低雄性Wistar大鼠接触香烟烟雾对肺部损伤的水平，并研究有效剂量的美国大叶Essanaana叶片可以降低肺部损伤的水平。用浸软法用70％乙醇提取6天大叶黄麻叶。将大鼠分成六组，每组四只动物：暴露于环境空气中（A组），暴露于香烟烟雾中（B组），暴露于香烟烟雾中并用45mg / kg体重，90mg / kg的美国大叶黄杨叶乙醇提取物处理bw，180mg / kg bw（C，D，E组），暴露于香烟烟雾中，并以18mg / kg bb维生素E（F组）处理。使用吸烟室每天用3支未过滤的香烟将所有大鼠治疗14天。 14天后，处死大鼠。取出右肺进行组织病理学检查。结果表明，剂量45mg / kg bw，90mg / kg bw，180mg / kg bw可降低肺对肺泡扩大，肺泡壁增厚和炎性细胞浸润的损害程度。与正常对照组相比，剂量为180mg / kg体重，作为减少肺损伤水平的有效剂量。
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